Tutorial: Guidelines for Single-Cell RT-qPCR

Tutorial: Guidelines for Single-Cell RT-qPCR

Blog Article

Reverse transcription quantitative PCR (RT-qPCR) has delivered significant insights in understanding the gene expression landscape.Thanks to its precision, sensitivity, flexibility, and cost effectiveness, BARLEY MALT RT-qPCR has also found utility in advanced single-cell analysis.Single-cell RT-qPCR now represents a well-established method, suitable for an efficient screening prior to single-cell RNA sequencing (scRNA-Seq) experiments, or, oppositely, for validation of hypotheses formulated from high-throughput approaches.Here, we aim to provide a comprehensive summary of the scRT-qPCR method by discussing the limitations of single-cell collection methods, describing the importance of reverse transcription, providing recommendations for the preamplification and primer design, and summarizing essential data processing steps.

With the detailed protocol attached in FLOR-ESSENCE the appendix, this tutorial provides a set of guidelines that allow any researcher to perform scRT-qPCR measurements of the highest standard.